Protein Characterization

Background Information

• Protein Assay Background

• PAGE Background

• Antibodies (hMDH1 /hMDH2) (His-Tag HRP)

Protocols

• 96 Well Plate Bradford Assay

• 1 ml Bradford Protein Assay

• SDS PAGE

• Native PAGE

• WesternBlot

• Fast Thermal Melt

• Heat Inactivation Protocol

Tutorials/ Videos

• Protein Quantitation Using the Bradford Assay

• Western Blot Video Tutorial

Teaching Files

• Instructions for SDS Page and Western Blot

Budget Saver Ideas

Something missing or do you have something to add? Email Josephprovost@sandiego.edu