MDH Enzyme Assay

Background Information

• Introduction to MDH Regulation and Kinetics

• Enzyme Kinetics Introduction

• Enzymes and Information from the study of enzyme kinetics

• Initial Rate Determination of Multi Substrate Enzymes

• Assay Hints and Tips

• Malate -> OAA colormetric assay (1) (2)

Protocols

• Continuous MDH Assay: 1 ml / 1.5 ml

• Range Finder

• 96 Well Plate Stop-Time Assay

• Determining Km and Vmax for MDH Using Initial Rates

Tutorials/ Videos

• Nuts and Bolts of the MDH Enzyme Assay (PPT)

• Analyzing Kinetic Data Using Excel

Teaching Files

• Handout for Gen Chem MDH CURE

• NADH and Buffer Spectroscopy Lab

  • USD Gen Chem Lab Example

  • USD Biochem Lab Example

• USD Biochem MDH Kinetics Enzyme Lab

• USD Biochem Hypothesis Design and Planning Lab

• Providence College Kinetics

Something missing or do you have something to add? Email Josephprovost@sandiego.edu